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Marywood University Biological Waste Disposal Standard Operating Procedure Page 1 2/18/2015. Marywood University Biological Waste Disposal Standard Operating Procedure Page 2. Techniques in Microbiology: A Student Handbook, Lammert, John M., Pearson Education, Inc. Abstract Review of: Techniques in Microbiology: a Student Handbook; John M. Lammert; (2007). Pearson Education, Inc., Upper Saddle River. Techniques Microbiology Student Handbook by John Lammert Techniques for Microbiology: A Student Handbook by John M. Lammert and a great selection of similar Used, New and Collectible Books available now at [PDF]GENERAL MICROBIOLOGY LAB Laboratory Manual & Workbook in MICROBIOLOGY, Applications to Patient Care, text. Lammert’s approach is visual and incorporates “voice balloons” that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory. AbeBooks.com: Techniques in Microbiology: A Student Handbook (116) by John M. Lammert and a great selection of similar New, Used and Collectible Books available now at great prices. Techniques in Microbiology: A Student Handbook John M. Lammert Pearson-Prentice Hall 2007 10th Edition 116 $28.00. Scientific and Investigative Techniques Stuart H. James, Jon J. HIS 499 A Student's Guide to History Jules R. Martin's Press 2014 12th Edition 444 $35.95.
Home / Periodicals / Journal of Microbiology & Biology Education / Volume 11, Issue 2 / Article A Review of Techniques in Microbiology: a Student Handbook Author: Padmapriya P.
Unit I — Safety first!
Safety in the microbiology laboratory
Universal precautions
Unit II — Culturing bacteria and aseptic techniques
Preparing culture media

Sterilization
The autoclave
Dry heat
Aseptic transfer of bacteria
Adjusting the gas burner
Preparing bacterial cultures in broth tubes and on agar slants
Transfer colony from plate to broth and to agar slant
Isolation of bacteria
Preparing a pour plate
Preparing a streak plate
Preparing a spread plate
Characteristic features of bacterial growth in broth and on agar
Maintenance and storage of stock cultures
Culturing anaerobic bacteria
Unit III — Visualizing bacteria
Effective use and responsible care of the light microscope
Measuring microscopic cells
Preparing a hanging-drop slide
Preparing a bacterial smear
Preparing a simple stain
Preparing a Gram stain
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Preparing an acid-fast stain
Preparing a negative stain
Preparing a capsule stain
Preparing an endospore stain
Unit IV — Enzyme-based tests for the identification of bacteria
Hydrolytic (digestive) enzymes
Starch hydrolysis
Casein hydrolysis
Gelatin hydrolysis
Lipid hydrolysis (tributyrin and spirit blue agar)
DNA hydrolysis
Utilization of carbohydrates
Fermentation of carbohydrates (Durham tube)
Methyl red test (mixed fermentation)
Voges-Proskauer test (butanediol fermentation)
Citrate utilization
Oxidation-fermentation (O-F) glucose test
Degradation of amino acids
Indole test
H2S production
Phenylalanine deamination
Decarboxylase test
Respiration tests
Catalase test
Oxidase test
Nitrate reduction
Techniques In Microbiology A Student Handbook Lammert Pdf To Excel Converter
Miscellaneous tests
Triple sugar iron (TSI) test

Urea hydrolysis
Litmus milk reactions
Motility assay
Coagulase production
Selective and/or differential media
Blood agar
Eosin methylene blue (EMB) agar
Mannitol-salt agar
MacConkey agar
Phenylethyl alcohol (PEA) agar
Unit V — Counting microbes
Direct microscopic counting with the Petroff-Hausser chamber
Preparing a standard plate count of bacteria
Using Turbidemetry to estimate cell density
Plaque assay for determining bacteriophage titer
Techniques In Microbiology A Student Handbook Lammert Pdf To Excel Online
Unit VI — Measuring effectiveness of antibacterial chemicals
Techniques In Microbiology A Student Handbook Lammert Pdf To Excel Pdf
Kirby-Bauer method for sensitivity of bacteria to antibacterial
medicines
Evaluating antibacterial chemicals: The disk-diffusion
method
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